Evaluation of the 6 possible redox states of Trx.

<p>In cells without stimulation (left three lanes), Trx is primarily in the reduced state, even with TR1 knocked-down and the C-terminal mutant TR1 expressed. With 1 mM diamide stimulation for 30 min (right three lanes), cells with endogenous TR1 demonstrate more reduced Trx than cells with endogenous TR1 knocked-down with siRNA.