Ndk inhibits EEA1 recruitment to phagosomes.

<p><b>A.</b> RAW cells were transfected with EEA1-GFP and thereafter allowed to ingest either control BSA- or Ndk-coated latex beads. The green signal shows presence of EEA1 on the phagosome containing control beads (upper panel), while the lack of signal around the phagosome (lower panel) shows diminished recruitment of EEA1 to Ndk-bead containing phagosomes. <b>B.</b> Quantification of the confocal data shown in panel A. <b>C.</b> Raw cells were transfected with 2xFYVE-GFP, and thereafter allowed to phagocytose either control BSA- or Ndk-coated latex beads. 2xFYVE is a specific marker for PI3P. The green signal seen in the upper control panel indicates an abundance of PI3P generated on the phagosome, while the lack of signal on Ndk-bead phagosomes indicate absence of PI3P. <b>D.</b> Quantification of confocal data shown in panel C. Values in B and D are the mean ± SD of phagosome colocalization with EEA1-GFP and 2xFYVE-GFP respectively in 50–80 cells from three independent experiments.