Western blot of serum dilutions developed for house sparrow IgY.
- Sarah K. Huber (351915)
- Jeb P. Owen (251940)
- Jennifer A. H. Koop (351914)
- Marisa O. King (251938)
- Peter R. Grant (368079)
- B. Rosemary Grant (368081)
- Dale H. Clayton (351918)
Publication date
February 2013
DOIAbstract
<p>Western blot of serum dilutions from Darwin's finch (DF), house sparrow and chicken using antibody markers developed for house sparrow IgY. Lane 1: DF serum 1∶10. Lane 2: DF serum 1∶20. Lane 3: house sparrow serum 1∶10. Lane 4: house sparrow serum 1∶20. Lane 5 chicken serum 1∶10. Lane 6 chicken serum 1∶20. Image indicates cross reactivity of house sparrow IgY detection antibody with Darwin's finch IgY. The lack of binding to chicken serum indicates no cross-reactivity with that species.</p
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