Effects of host-cell entry and inflammasome activation on miR-155 induction.

<p>(A) PBM were pretreated for 30 minutes with 5 µg/ml cytochalasin D (CytoD), infected with <i>F.n.</i> at an MOI of 50 for 6 hours, then relative miR-155 expression measured by qRT-PCR. (B) CFU assays were conducted in parallel with the samples from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008508#pone-0008508-g004" target="_blank">Figure 4A</a>. (C) Wild-type (WT) and caspase-1^−/− bone marrow-derived macrophages were infected with <i>F.n.</i> at an MOI of 50 for 8 hours and miR-155 was assayed by qRT-PCR. (D) ELISAs were done to measure IL-1β in supernatants from the samples in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008508#pone-0008508-g004" target="_blank">Figure 4C</a>. Graphs represent the mean and standard deviation from three independent infections. Data were analyzed by a paired Student <i>t</i> test. An asterisk (*) indicates a <i>p</i>-value<0.05.