NLS sequence of nucleolin is necessary for the nuclear translocation of Stat1.

<p>(A) For infection of monocytic murine M1 cells, a full-length (FL) and a NLS sequence deleted (ΔNLS) nucleolin cDNA from murine origin were used to generate lentiviral particles. (B) Three days post infection with FL(Ncl), ΔNLS(Ncl), or empty lentiviruses (con), M1 cells were stimulated for differentiation with TCM or left non-stimulated. Cells were fixed and permeabilized, and stained for Stat1 (monoclonal anti-Stat1 antibody and corresponding Alexa488-coupled secondary antibody, green colour). Simultaneous DNA labelling with DRAQ5 (red colour) was performed to visualize the nuclear compartment. The translocation of Stat1 into the nucleus was analyzed with a Leica TCS-SP2 AOBS confocal microscope.