Effect of small interfering (si) and scrambler (sc) RNA against p47phox on PGPC-dependent expression of p47phox in peripheral blood mononuclear cells.

<p>mRNA (A) was analyzed by quantitative Real-Time PCR. Normalized gene expression levels were given as the ratio between the mean value for the target gene and that for the beta-actin in each sample.. Figure shows a representative western blot analysis for p47phox and the average quantification obtained by densitometric analysis of all the samples (B). Data on Western blot analysis are expressed as the density ratio of target to control (Beta-actin) in arbitrary units ×10. Results are the means±SD of measurements performed in triplicate in six different occasions. *p<0.01 versus control; †p<0.01 versus PGPC; ‡<0.01 versus scRNA. PGPC = 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphorylcholine.