Effect of increasing amounts of vitamin C on PGPC-dependent activation of nuclear NF-kB and Nrf2 expression in peripheral blood mononuclear cells.

<p>PGPC was used at a concentration of 24 µM. NF-kB (A) was analyzed as described in materials and methods; Nrf2 mRNA (A) was analyzed by quantitative Real-Time PCR. Normalized gene expression levels were given as the ratio between the mean value for the target gene and that for the beta-actin in each sample. Figure shows a representative western blot analysis for Nrf2 and the average quantification obtained by densitometric analysis of all the samples (B). Data on Western blot analysis are expressed as the density ratio of target to control (Beta-actin) in arbitrary units ×10. Results are the means±SD of measurements performed in triplicate in six different occasions. *p<0.01 versus control; †p<0.01 versus 100 µM vitamin C; ‡p<0.01 versus 200 µM vitaminC; #p<0.01 versus 300 µM vitamin C. PGPC = 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphorylcholine; NF-kB = nuclear factor-kB, Nrf2 = Nrf2 = NF-E2-related factor 2.