Effect of 3-AB treatment on PARP-1 activation and PAR/NeuN, PAR/OX-42 immunocolocalization.

<p>(A) PARP-1 activation, evidenced by PAR immunostaining, was performed on coronal sections of rats submitted to 4 h and 24 h of ischemia and treated with vehicle or with 3-AB. A marked decrease in PAR formation was observable after 3-AB treatment. (B) Immunocolocalization for PAR and NeuN was performed at 4 h and 24 h of ischemia. Arrows show double-positive cells. Ischemia-induced PAR formation in neurons is predominant at 4 h. (C) Immunocolocalization for PAR and OX-42 was performed at 4 h and 24 h of ischemia. Arrows show double-positive cells. Ischemia induces PAR formation in OX-42 positive cells at 24 h only. Photomicrographs are representative of 6 to 11 animals at each time point. Scale bars = 50 µm.