Measurement of blastomere bulging during mitosis.

<p>A-C: The measurements (A) required to estimate blastomere bulging were performed on image stacks (C). The surface of S₁ (corresponding to half of an ellipsoid) and S₂ (corresponding to a truncated circle) was then calculated (B). An example is shown in C where both fluorescence and transmitted light images were used. D: Distribution of the «bulging» index. The Y axis corresponds to the percentage of blastomeres with a given bulging index (X axis).