Immunohistological localization of MSP1₄₂ in the villous stroma before and after perfusion.

<p>Panel (A,C) show before and panels (B,D) after perfusion with MSP1₄₂ in the absence of anti-MSP1 (No. 4; A,B) and following addition of anti-MSP1 (No. 5; C,D). The syncytiotrophoblastic membrane (STM) is shown in light green with strings of nuclei (blue). MSP1₄₂ has intense green staining only in the villous stroma of No. 5 following perfusion (indicated by arrows, D). <b>Panel E shows a hematoxylin and eosin stained section from the same placenta for reference. The labels are: IVS – intervillous space, FC – fetal capillary, STM - syncytiotrophoblastic membrane, FVS – fetal villous stroma. All sections are at 200× magnification</b>. The staining with pre-bleed sera from rats used for making the anti-rMSP1₄₂ polyclonal sera was identical to that shown in panels A and C (not shown).