Response of hepcidin transcript levels to hypoxia and chemical HIF stabilization in human hepatoma cells.

<p>(<b>A</b>) RNase protection assays (RPA) demonstrating hepcidin (HAMP) mRNA regulation and HIF target gene (IGFBP1 and ANGPTL4) induction in HepG2 cells after 16 h exposure to hypoxia (1% O₂), dimethyloxalylglycine (<i>abbreviated</i> DG <i>or</i> DMOG) or 2,2′dipyridyl (DP). (<b>B</b>) Hepcidin (HAMP), IGFBP1 and ANGPTL4 mRNA regulation in Huh7 cells. U6sn RNA served as loading control. Quantification was performed by phosphoimaging. Data are expressed as means±standard deviation (SD) of the indicated number of experiments; *<i>p</i><0.05, **<i>p</i><0.01 <i>vs.</i> unstimulated controls.