Wnt-pathway genetic lesions in MSI colon tumors and cell lines.

<p><i>NKD1, AXIN2, TCF7L2, CTNNB1</i>, and <i>APC</i> gene mutation status in 40 MSI-CRC tumors (top) and 11 cell lines (bottom). Key: −, no lesion; ins, nucleotide insertion; del, nucleotide deletion; /−, allelic deletion. For <i>NKD1</i> and <i>AXIN2</i>, mutation in indicated nucleotide is designated, while for <i>TCF7L2</i> the status of the poly(A) tract (A8–wild-type; A9–mutant) is designated. <i>CTNNB1</i> exon-3 was screened for activating mutations (M). For <i>APC</i>, tumors positive for protein-truncation (T) are indicated <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007982#pone.0007982-Powell1" target="_blank">[45]</a>. Presence (+) or absence (−) of <i>APC</i> and <i>CTNNB1</i> lesions in each cell line is as described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007982#pone.0007982-Gayet1" target="_blank">[48]</a>.