rHsp65 inoculation increased IFN-γ and IL-10 levels.

<p>Draining lymph nodes cells from either rHsp65 inoculated or control mice were harvested at day 21 and stimulated <i>in vitro</i> [10⁶ cells/ml] with 30 µg/ml IRBP [<i>A, C, E</i>] and 20 µg/ml <i>M. leprae</i> rHsp65 [<i>B, D, F</i>]. After 48 hours, IFN-γ [<i>A, B</i>], IL-10 [<i>C, D</i>] and IL-6 [<i>E, F</i>] levels were determined by ELISA. Data represent three independent experiments with n = 5 mice/group [*<i>p</i><0.05 versus control group; <i>t</i>-test analysis].