Comparison of plasmid yield using different purification and precipitation methods.

<p>Bacterial cultures of each plasmid were divided into three equal portions. The first was purified using the Qiagen Maxi prep kit, and the plasmid eluted from the column was precipitated by isopropyl alcohol after overnight incubation at −20°C using centrifugation (Lane A). The second portion was also purified using the Qiagen Maxi prep kit but the plasmid eluted from the column was isolated by glass syringe filter filtration (Lane B). For the last part of the culture, the plasmid was purified by 4 glass syringe filters and harvested by glass syringe filtration. (Lane C). The final volume of all plasmid preparations was 1 ml in TE (pH 8.0) and 5 ul was loaded on to a 0.8% TAE agarose gel.