Fragment analysis results.

<p>(A) Electropherograms obtained from gel electrophoresis of the multiplex amplification reaction. Data from four individuals are depicted. The relative fluorescence units (RFU) are indicated on the y-axis. The time interval for each of the electropherograms is approximately between 30 minutes (corresponding to fragments length slightly below 130 bp) and 43 minutes (equaling fragment lengths of about 215 bp). Each of the three investigated STRs produces a discrete peak cluster. The differences in the individuals' genotypes for this three-marker set are apparent. (B) Electropherograms derived from the simplex reactions of individual 4, displaying a clear correlation with the STR peak groups from the multiplex assay. RFU are shown on the y-axis. In this example, the TPOX locus is homozygous, whereas the two other loci – CSF1PO and D18S51 – are heterozygous, generating one and two DNA threads, respectively, in the TnT reaction.