Surface plasmon resonance shows that monoclonal antibody 15B3 selectively captures PG14 PrP aggregates from P3 fractions.

<p>Fractions P3 prepared from whole brains of Tg(PG14) or <i>Prnp</i>^0/0 mice were perfused for 5 min (bars) over the sensor surface of SPR chips on which antibody 15B3 (A) or 3F4 (B) had been immobilized. The sensorgrams (time course of the SPR signal in Resonance Units, RU) refer to the specific binding to the antibody (binding was negligible on sensor surfaces without antibody). Significant binding was detected when P3 fractions containing PG14 PrP (black line) were flowed over the 15B3-coated chip (A), but not on the 3F4-coated chip (B). No binding was observed when a P3 fraction from <i>Prnp</i>^0/0 mice was analyzed (gray line).