MLCs inhibit maDC-induced proliferation of CD4 T cells.

<p>CD4 T cells from DO11.10×C57BL/6 F1 hybrid mice were cocultured with maDCs and/or MLCs for 5 days. (a) Proliferation of CD4 T cells in the coculture system were demonstrated by CFSE dilution. (b) MLCs were added into the maDC/CD4 T coculture system simultaneously or 24 h after maDC coculture with CD4 T cells. Live CD4 T cells in the coculture system were counted by a flow cytometer. Data are presented as mean±s.d. of triplicate wells and representative of three independent experiments. (c) Fixed endothelial cells (EC) or endothelial supernatant were used to culture DC or neutralizing antibodies against TGF-β, M-CSF or VEGF were added into the endothelial-DC coculture system(5 ug/ml, each antibody), then the differentiated MLC were added into the maDC/T cell coculture system. 3 days later, the alive T cells were counted by FACS. ^**, <i>P</i><0.05. ^***, <i>P</i><0.01.