SDS-PAGE- and western blot-analysis of the recombinant enzymes of the <i>S. aureus</i> vitamin B1 metabolism.

<p>(A) SDS-PAGE of the affinity chromatography purified recombinant enzymes and (B) corresponding western blot analysis using a monoclonal anti-Strep antibody at a dilution of 1∶5,000 according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007656#s3" target="_blank">material and methods</a>. GTPase; TPK, thiamine pyrophosphokinase; TenA, thiaminase II; ThiM, THZ kinase; ThiD, HMP kinase; ThiE, TMP synthase. The arrow indicates the <i>Sa</i>ThiE protein.