Expression of miR-143 in prostate cancer.

<p><i>A</i>, Quantitative real-time PCR (QPCR) of miR-143, normalized to the amount of RNU48 target in prostate cancer cell lines. The relative levels of miRNA expression were measured by determining the ΔCt values of the indicated cell line versus PNT2 cells. Data are means ± SEM for three independent experiments. Statistical significance, here and in subsequent figures, *<0,05; **<0,01; ***<0,001. <i>B</i>, QPCR of miR-143, normalized to the amount of RNU48 target in prostate tissue samples. The relative levels of miRNA expression were measured by determining the ΔCt values of the indicated gleason prostate cancer versus normal prostate. Data are means ± SEM of eleven prostate samples for each group. <i>C</i>, Representative <i>in situ</i> hybridization staining of miR-143 in human non-tumor and tumor prostate tissue. TMA presents 40 prostate cancer tissues vs. 10 normal prostate tissues. (TMA, magnification, 400×).