Expression of EMT markers, E-cadherin and N-cadherin, in Twist positive cells in IPF lung tissue.

<p>(A) Dual immunofluorescence and (B) confocal microscopic image analyses of frozen lung sections of IPF lung stained with anti-E-cadherin (green) and anti-Twist (red) antibodies. E-cadherin staining was localized in the basolateral membrane in Twist negative cells (closed arrow). Twist positive cells showed weaker E-cadherin staining or cytoplasmic localization (open arrow). Magnification ×400 and ×1000 respectively. (C) Double immunofluorescence and (D) confocal microscropic image analyses of frozen lung sections of IPF lung stained with anti-N-cadherin (green) and anti-Twist (red) antibodies. Notice nuclear Twist positive cells showing N-cadherin (green) positive staining in the cytoplasm (open arrows). Some cells showed Twist positive staining but were negative for N-cadherin (close arrows). Magnification ×200 and ×1000 respectively. Stainings are representative of 3 independent assays in lung tissues from 3 IPF patients.