<i>reg4</i> gene amplification in pancreatic cancer and precancerous lesions measured by quantitative PCR.

<p>Homogeneous populations of cells were carefully microdissected using a Laser Capture Microdissection System. PanINs were graded 1 to 3 as a function of the importance of architectural and cytological abnormalities. DNA from pancreatic cancer samples obtained by endoscopic ultrasound (EUS)-guided fine needle aspiration was used. DNA from blood leucocytes was used as control. <i>reg4</i> gene copy number was determined by qPCR performed in triplicate and results were analyzed using the RealQuant software.