Influence of <i>DUN1</i>, <i>SML1</i>, or <i>CRT1</i> deletion on the mutation rates of <i>tsa1</i>Δ cells.

<p>The number of CAN^R (A) or 5FC^R (B) colonies on synthetic complete solid medium either lacking arginine but containing CAN (60 mg/L) or supplemented with 5FC (100 mg/L) was normalized with the total number of viable cells grown on the same solid medium without CAN or 5FC. The relative mutation rate of BY4741 cells (WT) was taken as 1.00. <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000697#s2" target="_blank">Results</a> represent the average from triplicate analysis of ten independent cultures. (C) Complementation of mutator phenotype in <i>tsa1</i>Δ <i>dun1</i>Δ cells by <i>TSA1</i> or <i>DUN1</i>. The rates of spontaneous CAN^R mutation were calculated as in (A). (D) Influence of ROS on mutation rates. Cells of the indicated genotypes logarithmically growing in YPD were subjected to treatment of H₂O₂ (0.6 mM, 15 min) before plating on synthetic complete solid medium lacking arginine and supplemented with CAN (60 mg/L). The rates of spontaneous CAN^R mutation were calculated as in (A).