M013 protein alone suppresses NF-κB activity and nuclear translocation of p65.

<p>(A) HeLa cells were co-transfected with equal amounts of pNF-κB-Luc and tk-Renilla-Luc along with pcDNA3.1M013L Myc/His or empty vector. At 48 h post-transfection, cells were either left untreated or treated with TNF (20 ng/ml) for 6 h and then assayed for both sea pansy and firefly luciferase activity. The presented values represent the average of three independent transfections. (B) Immunofluorescence and confocal microscopy analysis demonstrating the effect of M013 on TNF-induced nuclear translocation of NF-κB/p65 in HeLa cells. Mock (a–h) or M013L (i–p) transfected HeLa cells were labeled in the absence (a–d and i–l) or presence (e–h and m–p) of TNF treatment. (C) Immunofluorescence and confocal microscopy analysis demonstrating the effect of M013ΔPYD on TNF-induced translocation of NF-κB/p65 in HeLa cells. M013ΔPYD transfected HeLa cells were labeled in the absence (a–d) or presence (e–h) of TNF treatment.