Analysis of steatosis using synchrotron FTIR microspectroscopy.

<p>A) Optical image of steatotic hepatocytes containing steatotic vesicles (white star) and non-steatotic hepatocytes (black star). B) Averaged IR spectra recorded inside steatotic vesicles (upper spectrum in blue) or on non-steatotic hepatocytes (lower spectrum in red). The band corresponding to olefin (3000–3060 cm⁻¹) is labelled by a black arrow. C) Chemical imaging of some bands on the tissue section.