<p>(A) The wheat germ translation assay was programmed with artificial capped p33 mRNA carrying a poly(A) tail in the presence of CPZ, QC and DMSO as control. The radiolabeled p33 product was analyzed on SDS-PAGE. Each experiment was repeated three times. DMSO sample (0.03%) was chosen as 100%. (B) Similar in vitro translation assay was programmed with the uncapped wt TBSV gRNA, which does not carry a poly(A) tail.</p
<p>(A) Two biotinylated RNA templates were used for <i>in vitro</i> polyadenylation: an GAPDH mRNA t...
<p>(A) Krebs extract that was not nuclease-treated was programmed with Cap-Luc mRNAs (0.2 µg/ml) bea...
<p>COS-7 cells were stably transfected with shRNAs directed against COMP, and individual clones iden...
<p>(A) The in vitro band-shift assay included the radiolabeled RII(+)-SL sequence, which is present ...
<p>(A) The first in vitro replication assay contained a yeast cell-free extract, purified recombinan...
<p>(A) RNA-binding abilities of the GST-CP<sup>WT</sup> and GST-CP<sup>RA</sup> proteins as assessed...
<p>(A) Inhibition of protein synthesis by rCp-TSP13 in fat body organ culture system. Cycloheximide ...
<p>The 5′-capped p53-554 RNA was translated in RRL in the presence of <sup>35</sup>S-methionine and ...
AbstractExperiments were carried out to better characterize antisense control of translation, Result...
<p>(a) GA (■) inhibited the translation in a rabbit reticulocyte lysate more efficiently than cycloh...
<p>(A). Autoradiograph of translation products obtained from FMDV RNA annealed, or not, with the ind...
<p>Five early steps of TBSV replication <a href="http://www.plosone.org/article/info:doi/10.1371/jou...
<p>a. Dose dependent inhibition of luciferase mRNA translation by GST-SΔC in vitro (lanes 1–10) and ...
<p>200 ng of GST recombinant proteins (GST, GST-Sg4E-BP WT (WT), GST-Sg4E-BP YALA (YALA), GST-Sg4E-B...
<p>(A) Transiently transfected RL and FL reporters are not inhibited at transcript levels. HEK-293 c...
<p>(A) Two biotinylated RNA templates were used for <i>in vitro</i> polyadenylation: an GAPDH mRNA t...
<p>(A) Krebs extract that was not nuclease-treated was programmed with Cap-Luc mRNAs (0.2 µg/ml) bea...
<p>COS-7 cells were stably transfected with shRNAs directed against COMP, and individual clones iden...
<p>(A) The in vitro band-shift assay included the radiolabeled RII(+)-SL sequence, which is present ...
<p>(A) The first in vitro replication assay contained a yeast cell-free extract, purified recombinan...
<p>(A) RNA-binding abilities of the GST-CP<sup>WT</sup> and GST-CP<sup>RA</sup> proteins as assessed...
<p>(A) Inhibition of protein synthesis by rCp-TSP13 in fat body organ culture system. Cycloheximide ...
<p>The 5′-capped p53-554 RNA was translated in RRL in the presence of <sup>35</sup>S-methionine and ...
AbstractExperiments were carried out to better characterize antisense control of translation, Result...
<p>(a) GA (■) inhibited the translation in a rabbit reticulocyte lysate more efficiently than cycloh...
<p>(A). Autoradiograph of translation products obtained from FMDV RNA annealed, or not, with the ind...
<p>Five early steps of TBSV replication <a href="http://www.plosone.org/article/info:doi/10.1371/jou...
<p>a. Dose dependent inhibition of luciferase mRNA translation by GST-SΔC in vitro (lanes 1–10) and ...
<p>200 ng of GST recombinant proteins (GST, GST-Sg4E-BP WT (WT), GST-Sg4E-BP YALA (YALA), GST-Sg4E-B...
<p>(A) Transiently transfected RL and FL reporters are not inhibited at transcript levels. HEK-293 c...
<p>(A) Two biotinylated RNA templates were used for <i>in vitro</i> polyadenylation: an GAPDH mRNA t...
<p>(A) Krebs extract that was not nuclease-treated was programmed with Cap-Luc mRNAs (0.2 µg/ml) bea...
<p>COS-7 cells were stably transfected with shRNAs directed against COMP, and individual clones iden...