Specific enhancement of Tec1 sumoylation represses Tec1 activity.

<p><i>A</i>, transcription activity was measured in <i>tec1Δ</i> cells co-transformed with plasmids that express triple-FLAG-tagged Tec1, a Tec1-Ubc9 fusion, a Tec1^K54R-Ubc9 fusion, or the parent vector and a plasmid containing an invasive-specific <i>FRE</i> reporter (<i>TEC1</i> promoter, lacZ reporter).<i>B</i>, the same cells as in <i>panel A</i> were spotted onto solid YPD medium and after 2 days rubbed vigorously under a stream of water to detect invasive growth. <i>C</i>, transcription activity was measured in wild type cells transformed with plasmids that express a Tec1-Ubc9 fusion or the parent vector and a plasmid containing an invasive-specific <i>FRE</i> reporter as described in <i>panel A</i>. The data were statistically analyzed by <i>t</i> test with a <i>p</i> value of <0.05. <i>Error bars</i>, ± S.E. The data shown are representative of at least two independent experiments performed in triplicate.