<i>In situ</i> contractile analysis reveals trend towards force decrements, yet unaltered peak force and fatigue.

<p>Relative tetanic force production [in Newtons (N) per gram (g) of wet muscle mass] in the gastrocnemius/plantaris muscle group of high-fat diet (HFD) mice compared to control (CON) was (A) not different before (Pre) or after (Post) the fatigue protocol. (B) There was no difference between diets over all frequencies used to test force production pre-fatigue, however there was a significant main effect of diet post-fatigue. (C) Contractile force, relative to initial (% initial), throughout a 2 minute low-frequency fatigue protocol was not different between diet groups. Significance is represented by * vs. CON, p<0.005.