Exchange of H-2K^bOVA peptide is affected by MHC I tyrosine mutation on ΔY APCs.

<p>Splenocytes from K^bWT and ΔY mice were infected with <i>Listeria</i>-OVA between 0.001–0.1 MOI. Following 4 h infection, extracellular bacteria were removed and infected cells were incubated overnight. A. APCs were surface stained with a monoclonal antibody (mAb 25.D1.16) that specifically recognizes H-2K^b-OVA_(257–264) complexes and analysed by flow cytometry. Uninfected APCs or infected APCs pulsed with 1 µM OVA_(257–264) peptide for 1 h were used as negative and positive controls, respectively. B. Data is represented as a bar graph of the mean fluorescence intensity (MFI) of surface H-2K^bOVA_(257–264) complexes. Experiments were performed in triplicate and values are presented as average MFI plus SD with Student's <i>t</i> tests * <i>p</i><0.05.