Fluorescent immunocytochemical staining of Mos, Emi2, CyclinE and Cdk2 in elutriated PS and RS.

<p>A) Immunocytochemical localization of Mos and Emi2 in PS and RS. Mos and Emi2 are present in PS (a, a′), and in RS (b, b′). e, f (reaction with non immune rabbit IgG) and e′, f′ (reaction with non immune goat IgG) are the corresponding controls. c, d, g, h, c′, d′, g′ and h′ are the transmission pictures corresponding to a, b, e, f, a′, b′, e′ and f′ respectively. B): Immunocytochemical co-localization of Cyclin E and Cdk2 in PS and RS. Cyclin E (a, a′) and Cdk2 (b, b′) are present in PS and RS (c, c′: merge) respectively. d, d′, e and e′ are controls for Cdk2 (reaction with non immune mouse IgG) which were examined for green or red fluorescence. f, f′, g and g′ are controls for Cyclin E (reaction with non immune rabbit IgG) which were examined for red or green fluorescence. h and h′ are the transmission pictures corresponding to a, b, c and a′, b′, c′ respectively. i and i′ correspond to d, e and d′, e′; j and j′ correspond to f, g and f′, g′ respectively. All bars represent 10 µm.