Thaspine is a topoisomerase inhibitor.

<p>(A) Connectivity Map (CMAP) results after treatment with thaspine. The bar view is constructed from 6100 horizontal lines, each representing a single treatment and ordered according to their corresponding enrichment to the query signatures generated after thaspine treatment. Black horizontal lines in the barview represent the individual instances for ellipticine and camptothecin when the thaspine expression signature was used as query signature. Score according to the CMAP database; (B) inhibition of topoisomerase I activity: 1: plasmid +5U topoisomerase I; 2: plasmid; 3: plasmid +5U topoisomerase I+DMSO (1 µm); 4. Marker for nicked plasmid; 5: plasmid +5U topoisomerase I +50 µM thaspine; 6: plasmid +5U topoisomerase I +25 µM thaspine; 7: plasmid +5U topoisomerase I +10 µM thaspine; 8: plasmid + DMSO (1 µm). Topoisomerase I primarily induced nicked plasmid DNA, note the inhibition by thaspine. (C) inhibition of topoisomerase II activity: 1: plasmid +15U topoisomerase II; 2: plasmid; 3: plasmid +15U topoisomerase II + DMSO (1 µm); 4. Marker for nicked plasmid; 5: plasmid +15U topoisomerase II +50 µM thaspine; 6: plasmid +15U topoisomerase II +25 µM thaspine; 7: plasmid +15U topoisomerase II +10 µM thaspine; 8: plasmid + DMSO (1 µm). Topoisomerase II primarily induced nicked plasmid DNA, note the inhibition by thaspine.