<p>Cells were incubated with increasing doses of exogenous SMase. Concentration of active MMP-1 was determined in supernatants after 24h by ELISA. (*p<0.05, **p<0.01; n = 6)</p
<p>COS-7 cells transfected with indicated plasmids were pulse-labeled with [<sup>35</sup>S] methioni...
<p>After a 48(1–36), the MMP-2 (72 kd) total protein levels were assessed by western blot (A); mRNA ...
<p>Control A-10 cells, or cells stably expressing WT or 247R receptors were cultured in serum free m...
<p>Data are presented as relative increase after 6h and 24h in comparison to untreated control. (n =...
<p>Gelatin zymography (A) and western blot (B) that performed on cells treated with S1P for 48 hours...
<p>INS-1 cells were transfected with 1.6 µg MMP2 recombination plasmids and 4 µL Lipofectmine 2000 i...
<p>(<i>A</i>) MMP-1 secretion in supernatant after treatment with the indicated concentrations of CT...
<p>Fibroblasts were incubated with PEP (1 mg/ml) or left untreated (control) for 3 and 10 days befor...
<p>(A) C643 cells were transfected with negative control or with S1P<sub>2</sub> siRNA for 48 h, and...
<p>(A): Mock or NC1 α4(IV)-overexpressing cells were incubated for 48 h without FBS. MT1-MMP express...
<p>MDCK-T1 (A) and MDCK-T1D (B) cells in the absence or presence of control or MT1-MMP siRNAs were u...
<p><b>(A)</b> HUVECs were treated with PBS or NS1 for the indicated times; then, the supernatants we...
<p>Cells were treated with 1, 10, 100 ng/mL of HMGB1 for 18 h. (A). Cell culture medium was isolated...
<p>HuCCT1 cells were treated with 800 ng/ml ESPs or an equivalent volume of PBS, harvested after 24 ...
<p>Fibroblasts were incubated with PEP (1 mg/ml) or left untreated (control) for 3 and 10 days befor...
<p>COS-7 cells transfected with indicated plasmids were pulse-labeled with [<sup>35</sup>S] methioni...
<p>After a 48(1–36), the MMP-2 (72 kd) total protein levels were assessed by western blot (A); mRNA ...
<p>Control A-10 cells, or cells stably expressing WT or 247R receptors were cultured in serum free m...
<p>Data are presented as relative increase after 6h and 24h in comparison to untreated control. (n =...
<p>Gelatin zymography (A) and western blot (B) that performed on cells treated with S1P for 48 hours...
<p>INS-1 cells were transfected with 1.6 µg MMP2 recombination plasmids and 4 µL Lipofectmine 2000 i...
<p>(<i>A</i>) MMP-1 secretion in supernatant after treatment with the indicated concentrations of CT...
<p>Fibroblasts were incubated with PEP (1 mg/ml) or left untreated (control) for 3 and 10 days befor...
<p>(A) C643 cells were transfected with negative control or with S1P<sub>2</sub> siRNA for 48 h, and...
<p>(A): Mock or NC1 α4(IV)-overexpressing cells were incubated for 48 h without FBS. MT1-MMP express...
<p>MDCK-T1 (A) and MDCK-T1D (B) cells in the absence or presence of control or MT1-MMP siRNAs were u...
<p><b>(A)</b> HUVECs were treated with PBS or NS1 for the indicated times; then, the supernatants we...
<p>Cells were treated with 1, 10, 100 ng/mL of HMGB1 for 18 h. (A). Cell culture medium was isolated...
<p>HuCCT1 cells were treated with 800 ng/ml ESPs or an equivalent volume of PBS, harvested after 24 ...
<p>Fibroblasts were incubated with PEP (1 mg/ml) or left untreated (control) for 3 and 10 days befor...
<p>COS-7 cells transfected with indicated plasmids were pulse-labeled with [<sup>35</sup>S] methioni...
<p>After a 48(1–36), the MMP-2 (72 kd) total protein levels were assessed by western blot (A); mRNA ...
<p>Control A-10 cells, or cells stably expressing WT or 247R receptors were cultured in serum free m...
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