Extraction of cyclin B1 in various lysis buffers.

<p>Equal numbers of dissociated PC3 cells were solubilized in lysis buffer containing RIPA, Triton X-100 or SDS (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007064#s3" target="_blank">Materials and Methods</a>) and analyzed for cyclin B1 by Western blot (A). Increasing the SDS level to 23% did not release more cyclin B1 (B). There was some variability between lanes but, based on multiple gels, it was clear that cyclin B1 was readily extracted by all methods.