Adipocytes continue to differentiate after harvesting and replating post-induction.

<p>The 3T3-L1 preadipocytes were induced to undergo adipogenesis and harvested at day 3–4 post induction when lipid droplets were clearly visible. (A) Oil Red O staining of neutral lipids forty-eight hours after harvesting and replating. (B) The gene expression of adipocyte marker genes aP2, LPL, PPARγ, and adiponectin was measured via real-time PCR upon harvesting (Pre) and forty eight hours after (Post) the adipocytes were replated at 5.4×10⁴ cells/cm² (low) or 1.16×10⁵ cells/cm² (high) and compared to the expression of each gene in preadipocytes prior to induction (preAd). Expression of each gene was assayed in triplicate, normalized to cyclophilin B gene expression, and reported as the mean and standard deviation. The results are representative of experiments carried out twice independently.