<p>Optimum pH (A) metal dependence (B) and substrate specificity (C) of Det1 and YOR283w were measured by the release of inorganic phosphate as described in Experimental Procedures. σ, sodium acetate/acetic acid buffer; ⧫, Tris-acetate or Tris-HCl buffer; ν, CAPS/NaOH buffer.</p
A direct and continuous approach to determine simultaneously protein and phosphate con-centrations i...
Phosphatases are diverse groups of enzymes that deserve special attention because of their sig-nific...
The detection of small amounts (nanomoles) of inorganic phosphate has a great interest in biochemist...
<p>A. Purification of GST-YOR283w. Proteins were separated on a 12% SDS-polyacrylamide gel and stain...
<p>Phosphatase activity was measured using <i>p</i>-NPP. A) Magnesium dose-dependent increases of ac...
<p>A. Dusp15 phosphatase activity was assessed using the DiFMUP (6,8-difluoro-4-methyumbelliferyl ph...
<p>(a) Each of the indicated compounds (2 mM) was incubated with metal-removed TON_0340 (1 μM) at 37...
<p>(<b>A</b>) SDS-PAGE gel showing purified VPS29 and trimeric retromer proteins used for phosphatas...
<div><p>The detection of small amounts (nanomoles) of inorganic phosphate has a great interest in bi...
<p>The enzymatic activity of Fpy1p was determined as described under <i>Experimental Procedures</i>,...
205-210<span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-far...
<p>(<b>A</b>) <i>Para</i>-nitrophenyl phosphate (pNPP) is a generic phosphatase substrate whose deph...
Alkaline phosphatase activity in C57B1 mice thymic lymphomas has been characterized using pH optima,...
A, The specificity of the enzyme towards different phosphate (P)—containing compounds assayed at 1mM...
<p>Experimental conditions are detailed in Materials and Methods. Values are means ± SEM of two or t...
A direct and continuous approach to determine simultaneously protein and phosphate con-centrations i...
Phosphatases are diverse groups of enzymes that deserve special attention because of their sig-nific...
The detection of small amounts (nanomoles) of inorganic phosphate has a great interest in biochemist...
<p>A. Purification of GST-YOR283w. Proteins were separated on a 12% SDS-polyacrylamide gel and stain...
<p>Phosphatase activity was measured using <i>p</i>-NPP. A) Magnesium dose-dependent increases of ac...
<p>A. Dusp15 phosphatase activity was assessed using the DiFMUP (6,8-difluoro-4-methyumbelliferyl ph...
<p>(a) Each of the indicated compounds (2 mM) was incubated with metal-removed TON_0340 (1 μM) at 37...
<p>(<b>A</b>) SDS-PAGE gel showing purified VPS29 and trimeric retromer proteins used for phosphatas...
<div><p>The detection of small amounts (nanomoles) of inorganic phosphate has a great interest in bi...
<p>The enzymatic activity of Fpy1p was determined as described under <i>Experimental Procedures</i>,...
205-210<span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-far...
<p>(<b>A</b>) <i>Para</i>-nitrophenyl phosphate (pNPP) is a generic phosphatase substrate whose deph...
Alkaline phosphatase activity in C57B1 mice thymic lymphomas has been characterized using pH optima,...
A, The specificity of the enzyme towards different phosphate (P)—containing compounds assayed at 1mM...
<p>Experimental conditions are detailed in Materials and Methods. Values are means ± SEM of two or t...
A direct and continuous approach to determine simultaneously protein and phosphate con-centrations i...
Phosphatases are diverse groups of enzymes that deserve special attention because of their sig-nific...
The detection of small amounts (nanomoles) of inorganic phosphate has a great interest in biochemist...
DOI
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