P524-expanded CD4⁺CD25⁺ T cells prevented transfer of diabetes.

<p>Diabetic splenocytes (1×10⁷) were co-transferred with or without CD4⁺CD25⁺ T cells expanded by p509 (a), p524 (b), or p530 (c), to 4–8 wk old NOD.scid mice at the numbers indicated. In some cases, NOD.scid recipients receiving 6×10⁶ p524-expanded CD4⁺CD25⁺ T cells were injected with neutralizing antibodies to IL-10/TGF-β according to the regimens described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007034#s2" target="_blank">Materials and Methods</a>. Blood glucose was monitored for up to 8 wks. Differences in blood glucose levels between diabetic spleens alone and diabetic spleens plus 3×10⁶ p524-expanded CD4⁺CD25⁺ T cells were significant (P = 0.003), as were differences between diabetic spleens alone and diabetic spleens plus 6×10⁶ p524-expanded CD4⁺CD25⁺ T cells (P = 0.002). Cumulative data from three experiments are shown. The number of mice in each group is indicated in parentheses.