P524-expanded CD4⁺CD25⁺ T cells prevented/delayed the onset of diabetes in autoimmune diabetes-prone NOD mice.

<p>4–6 wk old NOD mice were transfused with CD4⁺CD25⁺ T cells (either 1×10⁶ (a) or 3×10⁶ (b) per mouse) expanded by p509, p524 or p530. Untreated littermates were used as controls. In some cases, NOD recipients receiving 3×10⁶ p524-expanded CD4⁺CD25⁺ T cells were injected with neutralizing antibodies to IL-10/TGF-β according to the regimens described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007034#s2" target="_blank">Materials and Methods</a>. Development of diabetes was monitored for over 25 wks and the cumulative incidence of diabetes was shown from two experiments. The difference between untreated controls and mice treated with 1×10⁶ p524-expanded CD4⁺CD25⁺ T cells was significant (P = 0.0036), as was that between untreated controls and mice treated with 3×10⁶ p524-expanded CD4⁺CD25⁺ T cells (P = 0.0015). The number of mice in each group is indicated in parentheses.