Cellular immune responses induced after immunization in CAF01 with or without a T helper epitope.

<p>IFN-γ ELISPOT responses against (A) Vif₁₀₁ CTL epitope and (B) PADRE Th epitope of splenocytes from HLA-A*0201 transgenic HHD mice after 10 days of s.c. immunization with Vif₁₀₁ and PADRE (grey bars, n = 5) or with Vif₁₀₁ alone (black bars, n = 5) and 5 days <i>in vitro</i> prestimulation with individual epitopes. The background of an unimmunized mouse is substracted. Specific ⁵¹Cr-release from target cells preloaded with Vif₁₀₁ after incubation with effector splenocytes from individual mice immunized with (C) Vif₁₀₁ and PADRE (grey, n = 5) or (D) with Vif₁₀₁ alone (black, n = 5). The percentage of specific lysis was calculated as 100×(experimental release-spontaneous release)/(total release-spontaneous release). Background lysis from an unimmunized mouse is shown (open circles). Significant levels are considered >10% lysis at a 50∶1 ratio of effector:target (E:T) cells. One representative experiment out of three. SFU, spot forming units.