Specificity and functional capacity of IDO5-specific T-cells assayed by ⁵¹Cr-release assays.

<p><i>(</i><i>a</i><i>),</i> Lysis of T2-cells pulsed with IDO5 peptide or an irrelevant peptide (the HLA-A2 high affinity binding epitope HIV-1 pol_476-484 (ILKEPVHGV)) by a T-cell clone (RBS35). <i>(</i><i>b</i><i>)</i>, Specific lysis by RBS35 of the HLA-A2⁺/IDO⁺ colon cancer cell line SW480 without or with the addition of the HLA-class I specific antibody W6/32, and lysis of the HLA-A2⁺/IDO^-colon cancer cell line HCT-116. All assays were performed in different E∶T ratios. <i>(</i><i>c</i><i>)</i> Histograms showing intracellular IDO expression in cancer cell lines. Data are representative of 3 experiments. Intracellular IDO expression was given by a one-tailed two sampled t-test comparing MFI_IDO (dark histograms) and MFI_{Isotype control} (light histograms), where MFI is the Mean Fluorescence Intensity. <i>Left:</i> HCT-116 (<i>p</i> = 0.300). <i>Right:</i> SW480 (<i>p</i> = 0.002).