<p>(A) Creation of 293T cell lines harboring stably downregulated β-TrCP1 and β-TrCP2 levels. Total RNA from 293T cell lines stably expressing the indicated shRNAmir constructs was extracted, and used to monitor β-TrCP1 and β-TrCP2 mRNA levels by real-time RT-PCR. The expression of the TBP cellular gene was used for normalization. The values of β-TrCP1 and β-TrCP2 measured in the presence of the control shRNAmir were given the arbitrary value of 100%. (B) Vpu requires β-TrCP2 to deplete tetherin from cells and antagonize its antiviral action. A Vpu-deleted HIV-1, or its Vpu-proficient counterpart, was transfected in duplicate in the indicated stable cell lines, in the presence of an HA-tagged tetherin plasmid (molar ratio of 2∶1 in favor of...
<p>(A) Co-expression of HIV-1 plasmids and wild-type (WT) N terminally tagged human tetherin and mea...
<p>(<b>A</b>) 293T/tetherin were transfected with NL4.3 wt or NL4.3 delVpu proviral plasmids in comb...
<p>293T were transfected with HA-tagged tetherin with or without Vpu, in the presence of either a Fl...
<p>(A) β-TrCP interaction motif is required for Vpu-induced tetherin depletion. HIV-1 deleted for th...
<p>(A) Vpu counteracts human but not murine tetherin antiviral activity. 293T cells were transfected...
<p>(A) The depletion of tetherin by Vpu correlates in a dose-dependent manner with its ability to bl...
<p>293T or COS cells were co-transfected with WT pNL4-3 or Vpu-defective (pNL4-3/delVpu) molecular c...
<p>(<b>A</b>) 293T cells were cotransfected with HIV-1 proviral (pNL4-3) plasmids expressing various...
<p>(<b>A</b>) 293T cells stably expressing Tetherin-HA were transduced with VSV-G pseudotyped HIV-1 ...
<p>(<b>A</b>) 293T/tetherin cells were transfected twice over 48 h with siRNA oligonucleotide direct...
<p>(A) 293T cells were co-transfected with 1 µg pNL4-3 WT or pNL4-3ΔVpu and 50 ng HA-tetherin or emp...
<p>(<b>A</b>) HT1080 cells stably expressing tetherin-HA were infected with VSV-G-pseudotyped HIV-1 ...
<p>(<b>A</b>) 293T/tetherin or 293T/tetherin Y6,8A mutant were infected with VSV-G-pseudotyped HIV-1...
<p>(<b>A</b>) Western blot analyses of the 293T cell lysates that were cotransfected with a Vpu-defi...
<p>(<b>A</b>) 293T cells were cotransfected with HIV-1 proviral (pNL4-3) plasmids expressing various...
<p>(A) Co-expression of HIV-1 plasmids and wild-type (WT) N terminally tagged human tetherin and mea...
<p>(<b>A</b>) 293T/tetherin were transfected with NL4.3 wt or NL4.3 delVpu proviral plasmids in comb...
<p>293T were transfected with HA-tagged tetherin with or without Vpu, in the presence of either a Fl...
<p>(A) β-TrCP interaction motif is required for Vpu-induced tetherin depletion. HIV-1 deleted for th...
<p>(A) Vpu counteracts human but not murine tetherin antiviral activity. 293T cells were transfected...
<p>(A) The depletion of tetherin by Vpu correlates in a dose-dependent manner with its ability to bl...
<p>293T or COS cells were co-transfected with WT pNL4-3 or Vpu-defective (pNL4-3/delVpu) molecular c...
<p>(<b>A</b>) 293T cells were cotransfected with HIV-1 proviral (pNL4-3) plasmids expressing various...
<p>(<b>A</b>) 293T cells stably expressing Tetherin-HA were transduced with VSV-G pseudotyped HIV-1 ...
<p>(<b>A</b>) 293T/tetherin cells were transfected twice over 48 h with siRNA oligonucleotide direct...
<p>(A) 293T cells were co-transfected with 1 µg pNL4-3 WT or pNL4-3ΔVpu and 50 ng HA-tetherin or emp...
<p>(<b>A</b>) HT1080 cells stably expressing tetherin-HA were infected with VSV-G-pseudotyped HIV-1 ...
<p>(<b>A</b>) 293T/tetherin or 293T/tetherin Y6,8A mutant were infected with VSV-G-pseudotyped HIV-1...
<p>(<b>A</b>) Western blot analyses of the 293T cell lysates that were cotransfected with a Vpu-defi...
<p>(<b>A</b>) 293T cells were cotransfected with HIV-1 proviral (pNL4-3) plasmids expressing various...
<p>(A) Co-expression of HIV-1 plasmids and wild-type (WT) N terminally tagged human tetherin and mea...
<p>(<b>A</b>) 293T/tetherin were transfected with NL4.3 wt or NL4.3 delVpu proviral plasmids in comb...
<p>293T were transfected with HA-tagged tetherin with or without Vpu, in the presence of either a Fl...