Immunoblotting analysis of the trypanosome antigens recognised during BALB/c mice infections and identification of potential immunoreactive proteins.

<p>Different trypanosome protein extracts: (3A,D) <i>Tbg</i>1122b total lysate, (3B) <i>Tbb</i>427 total lysate (T) and <i>Tbb</i>427 fractions containing the soluble proteins (F1) or cytoskeleton/membrane proteins (F2), (3C) <i>Tbb</i>427 cytoskeleton/membrane fraction (F2) were subjected to SDS-PAGE and tested by Western blotting against different sera from BALB/c (n = 4) mice infected with (10³) parasites, (3A–C): sera from <i>Tbg</i>945b (1), <i>Tbg</i>1122b (2), <i>Tbg</i>1135b (3), <i>Tbg</i>1135c (4); non-infected control mice (5) or (3D) antibodies specific for cytoskeleton or membrane proteins: rabbit polyclonal antibodies directed against ISG64 (6), ISG65 (7), ISG75 (8), mouse monoclonal antibodies recognizing PFR2 (9) or calflagin (10). A–C represents the results of one representative immunoblot out of 4 mice tested.