Plk1 expression and activity declines during rat cardiac myocyte development.

<p>Ventricular cardiac myocytes were isolated from Wistar rat hearts at various stages of development (embryonic day 18 [E18], 2, 5 and 10 days postnatal [P2, P5 and P10] and adult [250 g males]) and assessed for Plk1 mRNA (A, normalised to GAPDH) and protein (B, normalised to GAPDH) expression and activity (C). D) representative western blot of Plk1 expression. E) Representative kinase assay and corresponding protein expression (GAPDH was used as a loading control). Data is from three separate experiments, * p<0.05.