Karyosome formation during late spermatogenesis in <i>C. elegans</i>.

<p>Numbered nuclei highlighted by circles in (A) and (B) or arrows in (D) and (E) correspond to meiotic stages shown in (C). Pachytene (green), the condensation zone (teal), meiotic divisions (blue), post-meiotic region (purple). (A,B) DAPI-visualized nuclei in isolated and fixed male gonads (C) Enlarged images of DAPI-visualized nuclei in dissected and fixed male gonads. (D,E) A dissected and flattened non-fixed male gonad stained with the DNA dye Hoechst 33258 (D) and visualized by DIC optics (E). Scale bars represent 10 µm.