Detection of a putative schistosome PKA in parasite lysates.

<p>A, Western blotting of lysates from adult <i>S. mansoni</i> (Sm lysate) and two human cell lines (293FT, HT1080) using a polyclonal antibody specific for the human PKA-Cα subunit revealed the presence of a putative PKA-C subunit of the expected molecular weight of 40 kDa in the schistosome lysate. B, Putative PKA activity is detectable in lysate of adult <i>S. mansoni</i> worms using a fluorescence-based kinase assay. ▴, Sm lysate, • recombinant human PKA-Cα. C–F, Kinase activity was measured in kinase reactions containing Sm lysate (C and E) and recombinant human PKA-Cα (D and F) and the PKA inhibitors H-89 (C and D) and PKI 14–22 (E and F). ▴, inhibitor-treated; •, no inhibitor. B–F, each time point represents the mean of three biological replicates.