SmPKA-C encodes for an essential kinase activity.

<p>A, Electroporation of adult worms with 30 µg of SmPKA-C dsRNA (▪) resulted in 75% mortality while worms treated with control dsRNA (•) exhibited 100% survival. B, Three days after electroporation, SmPKA-C transcript levels were decreased in worms electroporated with 10 µg of SmPKA-C dsRNA, while transcript levels were unaffected in control dsRNA-treated worms, as determined by real-time PCR. C, 7 days after electroporation, SmPKA-C dsRNA-treated (•) and control dsRNA-treated worms (▴) were incubated for 2 h in 100 µM forskolin. PKA activity was significantly decreased after electroporation with 10 µg SmPKA-C dsRNA compared to control dsRNA-treated worms.