IFN-γ ELISPOT by CD3, CD4 or CD8 T cells stimulated with cyclin D1-DR1 or D1-1 peptides.

<p>(A) CD3 or CD4 T cells from a healthy donor (HLA-A0201 and DR.B1 0104/1104) were stimulated twice with human cyclin D1-derived peptides DR-1, as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006730#s2" target="_blank">Materials and Methods</a>. (B) CD3 or CD8 T cells from a healthy donor (HLA-A0201 and HLA-DR.B1 0801/0901) were stimulated three times with human cyclin D1-derived peptides D1-1. Stimulated T cells were challenged in IFN-γ ELISPOT assay with the stimulating peptide DR-1 or D1-1. The controls are: un-pulsed CD14+ cell as APCs, irrelevant peptides JAK2-DR for HLA-DR.B1 and EW for HLA-A0201. The results represent the average spots in triplicate cultures +/− SD. The figures show one representative data from multiple similar experiments.