Curcumin Induces HMW DNA Damage.

<p>Cells were treated for 72 hours with 25 µM curcumin or were left untreated. HMW DNA damage was studied using the CometAssay Reagent kit. Cells were resuspended in LMAgarose the mixture was placed on to CometSlides. Next, cells were lysed and DNA was released and allowed to unwind. After electrophoresis, slides were fixed and nuclei were stained with SYBR Green. Positive nuclei were analyzed using a fluorescence microscope and a filter for FITC. Nuclei were chosen in a random fashion by a blinded observer using a CCD-camera and a computer monitor. Comet tail length was quantified by a blinded observer using ImageJ image analysis software. A) Shown is a scatter plot were the tail length of the comet of all positive nuclei in the untreated and curcumin-treated group is depicted of 3 independent experiments. The solid horizontal line represents the mean. B) representative picture of a nucleus of an untreated cell. C) representative picture of a nucleus of a curcumin-treated cell, *** = p<0.001.