Figure 4

<p>(A) Rundown kinetics for chimeras. Representative current traces for human, murine receptors and chimeras showing currents after the first and 26^th application of 5-HT. Records were obtained from the same cell. All chimeras tested produced functional currents upon 5-HT activation. However, the currents through the different receptor chimeras showed a strong variation in kinetics and rundown. (B) Repeated 5-HT-applications (26 applications within 40 min) reduced peak currents (left) and charge (right). Differences were only marginally for peak currents, whereas analysis of charge showed more pronounced variation between all receptor types. (C) Charge variations between the different receptor types displayed a good correlation to kinetic parameters such as desensitization, plateau current and deactivation.