Expression of GFP.

<p>(A) Relative real-time RT-PCR analysis of GFP mRNA expression from newborn to maturity in founder transgenic pigs. The decline was significant in both K25-2 and K25-3 (p<0.001); (B) Western blots analysis of GFP protein from newborn to maturity in founder transgenic pigs; (C) Relative real-time RT-PCR analysis of GFP mRNA expression in transgenic fibroblast cells. The decline of mRNA from 20 to 90 days was significant (p<0.001); (D) Flow cytometry analysis of the percentage of phenotype positive cells. The decline of percentage of positive cells from 20 to 90 days was also significant (p<0.001); (E) Relative real-time RT-PCR analysis of GFP mRNA expression in various tissues of the transgenic pig. Variegation of GFP mRNA expression was shown in different tissues (p<0.001); (F) Western blots analysis of GFP protein in various tissues; (G) Relative real-time RT-PCR analysis of GFP mRNA expression in offspring transgenic pigs. The decline from founder to offspring was significant (p<0.001); (H) Western blots analysis of GFP protein in offspring transgenic pigs. Error bars denote standard deviations. Neg., non-transgenic pig.