Gene expression analysis in sorted PSA-NCAM subpopulations.

<p>Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of (a) renal stem/progenitor genes (<i>Six2, Cited1, Sall1, Wt1</i> and <i>Pax2</i>), (b) vimentin and E-cadherin genes expression in PSA-NCAM magnetically separated cells from HFK (15–19 weeks of gestation). Normalization was performed against control HPRT expression and RQ calculated relative to the PSA-NCAM⁻ fraction. Data were calculated as average±SD of at least 3 independent samples. ***P<0.001, *P<0.05 versus PSA-NCAM⁻. <i>Sall1</i> expression in NCAM⁺ EpCAM⁺ cells was near significance (p<0.059).