Liver engraftment of MSC in a mouse model of liver injury, after intrasplenic or intra-hepatic transplantation.

<p>A) When injected into the spleen of NOD/SCID mice, human adult and pediatric MSC engrafted and survived for 8 weeks in the spleen (a). Only few cells migrated to the liver with maximum 3 cells per high-power field observed (arrow, b). Anti-human vimentin Ab (panel a,b), Hoechst (panel c,d), overlay (lower panel e,f). B) When injected into liver parenchyma, MSC engrafted and high numbers of cells were detected (a,b). However, they retained their spindle shape morphology (b). Anti-human vimentin Ab (panel a,b), Hoechst (panel c,d), overlay (panel e,f). Higher magnification (b,d,f).